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MicroScale Thermophoresis (MST) is a technology for the interaction analysis of biomolecules. MicroScale Thermophoresis is the directed movement of particles in a microscopic temperature gradient. Any change of the hydration shell of biomolecules due to changes in their structure/conformation results in a relative change of the movement along the temperature gradient and is used to determine binding affinities. MST allows measurement of interactions directly in solution without the need of immobilization to a surface (immobilization-free technology). MicroScale Thermophoresis was developed by the NanoTemper Technologies GmbH, a German high tech company with headquarters in Munich. == Applications == Affinity * between any kind of biomolecules including proteins, DNA, RNA, peptides, small molecules, fragments and ions * for interactions with high molecular weight complexes, large molecule assemblies, even with liposomes, vesicles, nanodiscs, nanoparticles and viruses * in any buffer, including serum and cell lysate * in competition experiments (for example with subtrate and inhibitors) Stoichiometry Thermodynamic parameters Additional information * Sample property (homogeneity, aggregation, stability) * Multiple binding sites, cooperativity 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「microscale thermophoresis」の詳細全文を読む スポンサード リンク
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